KMID : 0355620070330020124
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Journal of Korean Association of Oral and Maxillofacial Surgeons 2007 Volume.33 No. 2 p.124 ~ p.130
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The effect of differential modulation of N-methyl-D-aspartate receptor on the proliferation of primary cultured normal human oral keratinocytes: DNA synthesis rate analysis
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Kim In-Soo
Paik Ki-Suk Chang Mi-Sook Lee Won Lee Seung-Pyo
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Abstract
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In the present study, I investigated the effects of N-methyl-D-aspartate (NMDA), arachidonic acid (AA), and Nitric Oxide Synthase Inhibitor (NOSI), alone or in combination, on the proliferation of cultured primary normal human oral keratinocytes (NHOK). The purpose of this study was therefore the preliminary study for the examination of the interaction between these agents and NHOK in order to elucidate the mechanisms by which epithelial growth and regeneration are regulated. NHOK were obtained from gingival tissue of 20 individuals aged 20 to 29, and third passage (P3) cells were used for this study. The DNA synthesis was measured by the BrdU assay. Addition of low concentration of AA (1 ?M) and high concentration of AA with NMDA group (NMDA+AA 10 ?M) made DNA synthesis rate increase significantly at the early stage. Adding NNA (10 ?M) affected DNA synthesis rate to increase significantly in 4 hours. At the early stage, DNA synthesis was significantly active in the NOS-I with NMDA groups than in the control and the NMDA-only group, while it didn¡¯t become statistically meaningful in 24 hours. AA 1 ?M and NNA 10 ?M may induce the proliferation of the NHOK independently and NOS-I may induce the proliferation of the NHOK with NMDA. These reactions might be related to the NMDA receptor in the cell and the change of the intracellular calcium ion concentration.
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KEYWORD
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N-methyl-D-aspartate, proliferation, Normal Human Oral Keratinocyte, Arachidonic acid, Nitric Oxide Synthase Inhibitor
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